Bollettino di zoologia agraria e bachicoltura

Vol. 34 No. 3 (2002): Serie2
Articoli

Bombyx mori transgenic lines for the induction of constitutive dsRNA interference of the clock gene period

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  • ALBERTO PICCIN,
  • SILVIA CAPPELLOZZA,
  • STEPHANE MOREAU,
  • PAOLA CISOTTO,
  • MIRKO PEGORARO,
  • GABRIELLA MAZZOTTA,
  • CLARA BENNA,
  • FEDERICA SANDRELLI,
  • MAURO ZORDAN,
  • LUCIANO CAPPELLOZZA,
  • RODOLFO COSTA
ALBERTO PICCIN Dipartimento di Biologia, Universita di Padova, Via U. Bassi 58/B, Padova
SILVIA CAPPELLOZZA Istituto Sperimentale Zoologia Agraria, Sezione Specializzata per la Bachicoltura, Via dei Colli 28, Padova
STEPHANE MOREAU Dipartimento di Biologia, Universita di Padova, Via U. Bassi 58/B, Padova
PAOLA CISOTTO Dipartimento di Biologia, Universita di Padova, Via U. Bassi 58/B, Padova
MIRKO PEGORARO Dipartimento di Biologia, Universita di Padova, Via U. Bassi 58/B, Padova
GABRIELLA MAZZOTTA Dipartimento di Biologia, Universita di Padova, Via U. Bassi 58/B, Padova
CLARA BENNA Dipartimento di Biologia, Universita di Padova, Via U. Bassi 58/B, Padova
FEDERICA SANDRELLI Dipartimento di Biologia, Universita di Padova, Via U. Bassi 58/B, Padova
MAURO ZORDAN Dipartimento di Biologia, Universita di Padova, Via U. Bassi 58/B, Padova
LUCIANO CAPPELLOZZA Istituto Sperimentale Zoologia Agraria, Sezione Specializzata per la Bachicoltura, Via dei Colli 28, Padova
RODOLFO COSTA Dipartimento di Biologia, Universita di Padova, Via U. Bassi 58/B, Padova

Published 2024-10-30

Keywords

  • Bombyx mori,
  • clock genes,
  • transgenesis,
  • dsRNA interference,
  • posttranscriptional gene silencing

How to Cite

Bombyx mori transgenic lines for the induction of constitutive dsRNA interference of the clock gene period. (2024). Bollettino Di Zoologia Agraria E Bachicoltura, 34(3). https://test-ojs-unimi-it.archicoop.it/index.php/bzab/article/view/27077

Abstract

The circadian clock of insects consists of interlocking transcriptional feedback
loops which are positively and negatively regulated by the complex interaction of
a number of "clock" genes and their protein products. One of these genes, period
(per), plays a central role in the core of the circadian oscillator of the dipteran
Drosophila melanogaster. Mutations in this gene lead to alterations in circadian
rhythmicity and developmental timing. In order to establish whether similar effects
can also be observed in other insects, we employed a construct suitable for the
embryonic transformation of the lepidopteran Bombyx mori. This construct was
engineered to allow specific ds RNA interference-induced post-transcriptional
gene silencing of the Bombyx per gene. A preliminary molecular characterization
of the resulting transformants shows that several independent transgenic lines
harboring variable numbers of transgenes were obtained.

PDF (Italian)